Generator

Part:BBa_K2068008

Designed by: Nithin Dharmaraj   Group: iGEM16_UCLA   (2016-09-13)


O3-33 Mutant aa173

In this gene we have introduced a Thrombin cleavage site(LVPRGS) to our Wild Type O3-33 gene(BBa_K2068005). This gene will function exactly like the O3-33 gene as it will code for a single subunit of a self assembling 24 subunit protein cage. But, the subunits that form will include a Thrombin cleavage site and the resulting cage will be susceptible to controlled disassembly using a Thrombin protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 354
    Illegal AgeI site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None