Generator

Part:BBa_K2068005:Design

Designed by: Nithin Dharmaraj   Group: iGEM16_UCLA   (2016-09-13)


O3-33 Wild Type


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 591
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 284
    Illegal AgeI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This cage was computationally designed. The two steps that were crucial in the design of the cage were 1) symmetrical docking of protein building blocks in a target symmetric architecture 2) design of low energy protein-protein interfaces between the protein building blocks to drive self assembly.


Source

This cage is originally designed by Dr.Neil King.

References