Generator
Part:BBa_K2068005:Design
Designed by: Nithin Dharmaraj Group: iGEM16_UCLA (2016-09-13)
O3-33 Wild Type
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 591
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 284
Illegal AgeI site found at 213 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This cage was computationally designed. The two steps that were crucial in the design of the cage were 1) symmetrical docking of protein building blocks in a target symmetric architecture 2) design of low energy protein-protein interfaces between the protein building blocks to drive self assembly.
Source
This cage is originally designed by Dr.Neil King.