Composite

Part:BBa_K2066055:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


T7 Promoter without RiboJ Promoter Characterization Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is meant for use in Promoter Characterization and RBS Characterization subprojects for WM iGEM 2016. It should be compared against K2066054, which contains the RiboJ sequence.

Source

The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The broccoli sequence is from https://www.addgene.org/66843/. The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.

References