Composite

Part:BBa_K2066039:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


Sigma54 Enhancer 57S with DT, UNS Standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 111
    Illegal NheI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed for Gibson Assembly from the 57S plasmid from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper, with a UNS backbone to create K2066038. Note that this part has the B0015 double terminator, despite the fact that the original 57S plasmid did not have one after the mCherry + NRII coding regions.

Source

Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper

UNS sequences from Torella et. al 2013 "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly"

References