Composite

Part:BBa_K2066001:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-07-12)


UNS Standard Adapter with Red Chromoprotein.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to maintain a standard that allowed us an easy and consistent way to PCR and gibson clone our samples, which we did not find in the Prefix and Suffix. PCRs and Gibson assemblies using USN 2 and 3 primers have been very effective.

We chose to use the eFarRed chromoprotein developed by Uppsala iGEM 2012 to have an easily-visible indicator of the presence of the construct.


Source

The UNS2 and UNS3 sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. Other sequences are from the iGEM registry.

References