Composite
Part:BBa_K2064002:Design
Designed by: Dragos S. Chiriac Group: iGEM16_Queens_Canada (2016-10-21)
TyrB Acceptor and Donor COM
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 64
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 32
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 243
Illegal BsaI.rc site found at 222
Illegal SapI.rc site found at 282
Design Notes
Xba1 cut site removed, originally located downstream of the promoter
Source
Synthetic, codon optimized for E. coli