Composite

Part:BBa_K2064001:Design

Designed by: Dragos S. Chiriac   Group: iGEM16_Queens_Canada   (2016-10-21)


TyrA Donor COM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 32
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 195
    Illegal BsaI.rc site found at 174


Design Notes

Xba1 cut site removed from the region downstream of the promoter


Source

Synthetic, codon optimized for E. coli

References