RNA
Part:BBa_K206031:Design
Designed by: Alex Ng Group: iGEM09_British_Columbia (2009-06-06)
Key 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
taR12 sequence from Isaacs et al. (1) with the following modifications: a. removal of last 6 nucleotides from the 3' end, to reduce probability of unwanted binding from complimentary scars b. changing of nucleotide 13 from G to U, to allow complementarity with the suffix scar to block an exposed rbs
Source
1) Isaacs, FJ, Dwyer, DJ, Ding, C, Pervouchine, DD, Cantor, CR, and Collins, JJ. Engineered riboregulators enable post-transcriptional control of gene expression. 2004. Nature Biotechnology 22(7):841-847.