Composite

Part:BBa_K2057005:Design

Designed by: José Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


Expression of gfp under the regulation of promoter ChiA74


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1218


Design Notes

We need to amplify the promoter chiA74 as it was not available in the biobricks.


Source

Promoter chiA74 is part of the chiA74 gene locate on the chromosome of a strain of Bacillus thuringiensis. The gfp is obtained from the biobrick BBa_E0040

References

(i) Barboza-Corona JE, Delgadillo-Ángeles JL, Castañeda-Ramíırez JC, Barboza-PĂ©rez UE, Casados-VĂĄzquez LE, Bideshi DK and del RincĂłn-Castro MC (2014) Bacillus thuringiensis subsp. kurstaki HD1 as a factory to synthesize alkali-labile ChiA74∆sp chitinase inclusions, Cry crystalsand spores for applied use. Microb Cell Fact 13, 15. doi: 10.1186/1475-2859-13-15 (ii) Barboza Corona JE, Nieto-Mazzocco E, VelĂĄzquez-Robledo R, Salcedo-Hernandez R, Bautista M, JimĂ©nez B, Ibarra JE (2003) Cloning, sequencing and expression of the chitinase chiA74 from Bacillus thuringiensis. Appl Environ Microbiol. 2003 Feb;69(2):1023-9.