Part:BBa_K2054000:Experience

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Since the plasmids are made, the next stage we plan to take is to achieve the self-assembly of our desired tetrahedral nanostructure in vivo, then extract the product(s) out, using TRIzol® Reagent protocol (Thermo Fisher) for analyses. With reference to the same article, Elbaz stated that replacing the Zymo-SpinTM IC columns by the Zymo-SpinTM IIC Columns will enhance the binding efficiency. Elbaz also successfully purify a 2D structure, but it was quickly degraded. So our future works include strategies that hope to first get our desired 3D nanostructure assembled inside the cell, which is stable and functional for real-world diagnostic applications.

The following schematic diagram (fig 1a extracted from the article) summarizes the idea of our project idea.

source: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.

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