Coding

Part:BBa_K2045005:Design

Designed by: Thiban Navaratnarajah   Group: iGEM16_Concordia   (2016-10-14)


pTac-MelA(P334S)-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1224
    Illegal BsaI.rc site found at 529


Design Notes

pTac promoter and T1 terminator were chosen to ensure that this part can be easily used since pTAC and T1 terminators are the most common promoter and terminators used.


Source

Rhizobium etli. genome Santos CNS, Stephanopoulos G. Melanin-Based High-Throughput Screen for l-Tyrosine Production in Escherichia coli . Applied and Environmental Microbiology. 2008;74(4):1190-1197. doi:10.1128/AEM.02448-07.

References