Composite

Part:BBa_K2041010:Design

Designed by: ZHOU CHUANGUI   Group: iGEM16_BIT   (2016-10-14)


plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 418
    Illegal NheI site found at 3082
    Illegal NheI site found at 3105
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4041
    Illegal BamHI site found at 692
    Illegal XhoI site found at 779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1331
    Illegal NgoMIV site found at 1418
    Illegal AgeI site found at 468
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1526
    Illegal BsaI.rc site found at 1872
    Illegal BsaI.rc site found at 2975
    Illegal BsaI.rc site found at 4720


Design Notes

This is a composite part including Bxb1 rocombinase coding region encoded in part BBa_K907000,it also includes the recognition site of Bxb1 rocombinase and an amplifying system.



Source

All of these parts are from Kit Plate of iGEM foundation.


References