Part:BBa_K2040119:Design

mRFP1 + TtrpC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 715
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1351
  Illegal AgeI site found at 555
  Illegal AgeI site found at 667
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1065
  

Design Notes

Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.

Source

mRFP1 orignated from Discosoma striata; TtrpC was isolated from pAN7-1.