Regulatory

Part:BBa_K2040100:Design

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-11)


PMcl1, hemolymph inducible promoter from Metarhizium anisopliae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1673
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 853
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1179
    Illegal SapI site found at 1130


Design Notes

Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.

Source

It was isolated from the genomic DNA of Metarhizium anisopliae.

References

Metarhizium anisopliae collagen-like protein Mcl1 (Mcl1) gene, complete cds.(DQ238489.1) http://www.ebi.ac.uk/ena/data/view/DQ238489

Metarhizium anisopliae collagen-like protein Mcl1 (Mcl1) mRNA, complete cds.(DQ238488.1) http://www.ebi.ac.uk/ena/data/view/DQ238488