Composite

Part:BBa_K2039001:Design

Designed by: Coline Sivelle   Group: iGEM16_Paris_Saclay   (2016-10-13)


Part expressing the dimerization protein FRB* and GFP11 (subunit of tripartite GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutations introduced are : T2098L, K2095P, W2101F.


Source

Gblock

References