Composite

Part:BBa_K2036032:Design

Designed by: Zhi Zeng   Group: iGEM16_HUST-China   (2016-09-24)


CII-TT-pRE-RBS-GFP-LVAssrAtag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1142


Design Notes

CII can serve as transcriptional activator to initiate pRE.

Source

CII and pRE are both from bacteriophage lambda.

References

Marr M T, Roberts J W, Brown S E, et al. Interactions among CII protein, RNA polymerase and the λ PRE promoter: contacts between RNA polymerase and the–35 region of PRE are identical in the presence and absence of CII protein[J]. Nucleic acids research, 2004, 32(3): 1083-1090.