Composite

Part:BBa_K2036022:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)


patp2-RBS-CI-TT-pR-RBS-CIII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

With this two pormoter design, lactose an induce downstream gene's expression,and CII protein can form a positive feedback by activate pRE so, the circuit can fastly reach a stable state for Cro expression.


Source

placm is a mutant lac promoter which has been deleted a illegal restriction site. pRE, Cro, CII are from bacteriaphage lambda.

References