Coding

Part:BBa_K2036001:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)


CIII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It can inhibit Ftsh to degrade CII,thus protecting CII to initiate pRE and express downstream gene. One can directly insert CIII into MCS of a expression plasmid which contains promoter and terminator. And considering that Ftsh normally exists in E.coli, sometimes we can try over express CIII to guarantee CII’s function.

Source

come from bacteriaphage lambda operon

References

Halder S, Datta A B, Parrack P. Probing the antiprotease activity of λCIII, an inhibitor of the Escherichia coli metalloprotease HflB (FtsH)[J]. Journal of bacteriology, 2007, 189(22): 8130-8138.