Plasmid

Part:BBa_K2033011:Design

Designed by: Jimmy Xu   Group: iGEM16_Arizona_State   (2016-10-12)


N-Acyl Homoserine Lactone (AHL) Modular Sender Vector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A suitable RBS was difficult to find. The binding affinity either resulted in overflow readings or minimal readings. The RBS B0034 was found to produce appropriate reading levels.


Source

This part was designed with the help of Dr. Karmella Haynes, Ryan Muller, Cassandra Barrett, and Rene Davis.

References