Coding

Part:BBa_K2032009:Design

Designed by: Chun Mun Loke   Group: iGEM16_UMaryland   (2016-10-19)


MMOY/Z+TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1712
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1712
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1712
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used Gibson assembly


Source

MMO subunits from M. Capsulatus, commonly used terminators from iGEM registry (E. coli)

References