Regulatory
Part:BBa_K203109
Designed by: Lars Velten, Simon Haas, Hannah Meyer, Anne Rademacher, Hannah Uckelmann and Corinna Hiller Group: iGEM09_Heidelberg (2009-10-15)
Constitutive promoter; 2.5 REU
A constitutive promoter from the Heidelberg 2009 library of promoters for use in mammalian cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.
This promoter corresponds to clone S4. We confirmed the initial measurement by an independet technique (image analysis). Its expression strength is approx 2,5 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU].
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Categories
Parameters
None |