Plasmid

Part:BBa_K203099

Designed by: Lars Velten, Simon Haas, Anne Rademacher, Hannah Meyer and Michael Bartoschek   Group: iGEM09_Heidelberg   (2009-10-15)

pSMB_REFERENCE

Can be used for normalizations to transfection efficiency during promoter measurements in mammalian cells, if Part:BBa_K203100 pSMB_MEASURE is used for measurements.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 171
    Illegal XbaI site found at 1632
    Illegal SpeI site found at 185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal prefix found in sequence at 171
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 171
    Illegal BglII site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 171
    Illegal XbaI site found at 1632
    Illegal SpeI site found at 185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 171
    Illegal XbaI site found at 1632
    Illegal SpeI site found at 185
    Illegal NgoMIV site found at 1492
    Illegal NgoMIV site found at 2755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4283
    Illegal SapI site found at 3200


[edit]
Categories
Parameters
None