Plasmid_Backbone

Part:BBa_K2029015:Design

Designed by: Max Schelski, Niklas Schmacke, Cathleen Hagemann, Sophia Mädler   Group: iGEM16_UBonn_HBRS   (2016-10-14)


pSBbs1AK1 (replicative Bacillus subtilis expression vector)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5414
    Illegal XbaI site found at 5429
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5414
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 5420
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5414
    Illegal BglII site found at 4205
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5414
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5429
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal AgeI site found at 3816
    Illegal AgeI site found at 4778
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1176
    Illegal BsaI.rc site found at 2531
    Illegal BsaI.rc site found at 5047
    Illegal SapI.rc site found at 4029


Design Notes

A double resistance for selection in both B.subtilis and E.coli was needed


Source

pSB1A3 and a plasmid from Japan

References