Translational_Unit

Part:BBa_K2029013:Design

Designed by: Bui Hoang Duy   Group: iGEM16_UBonn_HBRS   (2016-10-14)


nprb_EstC2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 885
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1199


Design Notes

Designed for use in Bacillus subtilis; C-terminal 6His-tag added for convenient purification


Source

Enzyme coding gene: https://www.ncbi.nlm.nih.gov/nucleotide/283462288?report=genbank&log$=nuclalign&blast_rank=1&RID=XKTEYKV5014&from=7&to=1710

References

https://parts.igem.org/Part:BBa_K2029000 https://parts.igem.org/Part:BBa_K2029007