Coding

Part:BBa_K2027056:Design

Designed by: Gordon Sun   Group: iGEM16_Stanford-Brown   (2016-10-24)


E. coli DXS Synthase Enzyme cds (codon optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 257
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 134
    Illegal SapI.rc site found at 688


Design Notes

This sequence has been codon optimized for expression in E. coli.

The stop codon has been removed from this sequence to allow for C terminus concatenation with tags.

No further modifications were made.

Source

Sequence is sourced from Zhao Y, Yang J, Qin B, et al. Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway. Appl Microbiol Biotechnol. 2011;90(6):1915-22. (http://www.ecogene.org/gene/EG13612/dnasequence?q=gene/EG13612/dnasequence&sites[0]=BamHI&sites[1]=EcoRI&sites[2]=HindIII&left_end=433315&right_end=443315&type=fasta)

EcoGene: EG13612

References