Composite

Part:BBa_K2027012:Design

Designed by: Michael Becich   Group: iGEM16_Stanford-Brown   (2016-10-08)


Mutant Feedback-Resistant Version of MelA Tyrosinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1379
    Illegal AgeI site found at 1661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1231
    Illegal BsaI.rc site found at 25
    Illegal BsaI.rc site found at 536


Design Notes

Part BBa_K274001 was codon-optimized and we took into the considerations from Gosset (https://www.ncbi.nlm.nih.gov/pubmed/24225202) in order to create a feedback-resistant version of the tyrosinase in order to overproduce melanin. The part was synthesized by IDT with tails to 2-part Gibson Assembly into pSB1C3.


Sequence Verification

Source

https://www.ncbi.nlm.nih.gov/pubmed/24225202 http://partsregistry.org/Part:BBa_K274001

References