Device

Part:BBa_K2018036:Design

Designed by: Joel Vej-Nielsen   Group: iGEM16_SDU-Denmark   (2016-06-24)


phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 980
    Illegal BglII site found at 1805
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 286
    Illegal NgoMIV site found at 357
    Illegal NgoMIV site found at 957
    Illegal NgoMIV site found at 1269
    Illegal NgoMIV site found at 1548
    Illegal NgoMIV site found at 2200
    Illegal NgoMIV site found at 2222
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4066


Design Notes

None


Source

E. coli & R. eutropha Tokyo tech

References