Composite

Part:BBa_K2015013:Design

Designed by: Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


GFP and Zip-Up linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 913
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 746


Design Notes

The part contains restriction sites such as BglII, HindIII, and ClaI. If you cut BglII site, you can ligate with BBa_K2015000.

You can amplify this fragment following two primers.
Forward: GGGAGATCTTGTTGGGAGGGC(starting from BglII site)
Reverse: CCCAGATCTGCCACCGCC(starting from BglII site)


Source

gBlocks® Gene Fragments from IDT

References