Composite

Part:BBa_K2015012:Experience

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


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Applications of BBa_K2015012

We combined BBa_K2015011 and mRFP(BBa_E1010) to be determined. You can observe red color colonies expressing mRFP. K2015011 contains plac(R0011) and is regulated by IPTG.
We prepared two samples, IPTG(+), IPTG(-). Fig. 1. shows the pictures of the two plates. The colonies with IPTG express Red color.

T--HokkaidoU_Japan--K2015012-RFP.png
Fig. 1. Change of expression by IPTG induction
left: not induced, right: induced



The results indicate the fact that IPTG(-) does not express mRFP, but IPTG(+) can express it. In other words, a LacI expression unit can strictly regulate plac in pSB1C3.

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