Coding

Part:BBa_K2015009:Design

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


P11-4 for multimer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
    Illegal BamHI site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


Design Notes

Design Notes[edit]

This sequence contains two BamHI sites to replace enzymes.
You cannot cut this part by NdeI because GFP contains it.

You can amplify this fragment following two primers.
Forward: GGGTCTAGAATGGGTGGGTGCG(starting from XbaI site)
Reverse: CCCACTAGTTATTAGCCACCGCATCC(starting from SpeI site)


Source

gBlocks® Gene Fragments from IDT


References

[1] Riley JM, Aggeli A, Koopmans RJ, McPherson MJ, (2008) Bioproduction and Characterization of a pH Responsive Self-Assembling Peptide. Biotechnol Bioeng. 103(2): 241-51
[2] Kyle　S, Aggeli A,Ingham E, McPhersona MJ, (2010) Recombinant self-assembling peptides as biomaterials for tissue engineering．Biomaterials. 31(36): 9395-9405
[3] Prakash A,Parsons SJ, Kyle S, McPherson MJ (2012) Recombinant production of self-assembling β-structured peptides using SUMO as a fusion partner. Microbial Cell Factories. 2012, 11:92.