Coding

Part:BBa_K2015008:Design

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


RADA for multimer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 82
    Illegal BamHI site found at 802
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731


Design Notes

This sequence contains two BamHI sites to replace enzymes.
You cannot cut this part by NdeI because GFP contains it.

You can amplify this fragment following two primers.
Forward: GGGTCTAGAATGGGTGGGTGCG(starting from XbaI site)
Reverse: CCCACTAGTTATTAGCCACCGCATCC(starting from SpeI site)



Source

gBlocks® Gene Fragments from IDT

References

[1] Liang P, Xiong J, Zhao L, Xu Y, Zhao J, Liu Q (2015) Recombinant self-assembling 16-residue peptide nanofiber scaffolds for neuronal axonal outgrowth. Eng. Life sci. 15(1): 152-158
[2] Nune M, Kumaraswamy P, Krishnan UM, Sethuraman S, (2013) Self-Assembling Peptide Nanofibrous Scaffolds for Tissue Engineering: Novel Approaches and Strategies for Effective Functional Regeneration. Curr. protein pept. sc. 14: 70-84.
[3] Reed CD, Barnard GC, Anderson EB, Klein LT, Gerngross TU (2006) Production and puriWcation of self-assembling peptides in Ralstonia eutropha. Protein expres. purif. 46: 179-188.
[4] Zhang S, Behnke RE, Zhao X, Spirio L, PuraMatrix: Self-assembling Peptide Nanofiber Scaffolds. Tissue eng.