![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2014012:Design
Designed by: Daria Niewiadomska Group: iGEM16_UAM_Poznan (2016-10-12)
pxylF-xylA->sfGFP_W
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 437
Illegal SpeI site found at 572
Illegal SpeI site found at 749 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 437
Illegal SpeI site found at 572
Illegal SpeI site found at 749 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 153
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 437
Illegal SpeI site found at 572
Illegal SpeI site found at 749 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 437
Illegal SpeI site found at 572
Illegal SpeI site found at 749 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We were looking for the best method to optimize gene expression in E.coli.
Source
The xylF-xylA promoter is a modified version of xylR-xylF-xylA promoter present in E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence.