Generator

Part:BBa_K2014010:Design

Designed by: Daria Niewiadomska   Group: iGEM16_UAM_Poznan   (2016-10-12)


pxylS-E1_5'UTR->sfGFP_W


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 203
    Illegal SpeI site found at 338
    Illegal SpeI site found at 515
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 203
    Illegal SpeI site found at 338
    Illegal SpeI site found at 515
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 203
    Illegal SpeI site found at 338
    Illegal SpeI site found at 515
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 203
    Illegal SpeI site found at 338
    Illegal SpeI site found at 515
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were looking for the best method to optimize gene expression in E.coli.


Source

We used one of the sfGFP permutation, with optimized codon sequence.


References