Generator

Part:BBa_K2014003:Design

Designed by: Daria Niewiadomska   Group: iGEM16_UAM_Poznan   (2016-10-12)


pBAD-M5'UTR->sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 53
    Illegal SapI.rc site found at 352


Design Notes

We designed shortened arabinose induced promoter with modified 5'UTR to provide higher expression level.


Source

Arabinose induced promoter pBAD is a fragment of E.coli K-12 genome. The original 5'UTR region is replaced by synthetic unstructured sequence with a well positioned RBS previously tested on melibiose promoter from E.coli K-12 in 2015.

References