Generator
Part:BBa_K2014003:Design
Designed by: Daria Niewiadomska Group: iGEM16_UAM_Poznan (2016-10-12)
pBAD-M5'UTR->sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 236
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 53
Illegal SapI.rc site found at 352
Design Notes
We designed shortened arabinose induced promoter with modified 5'UTR to provide higher expression level.
Source
Arabinose induced promoter pBAD is a fragment of E.coli K-12 genome. The original 5'UTR region is replaced by synthetic unstructured sequence with a well positioned RBS previously tested on melibiose promoter from E.coli K-12 in 2015.