![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2014002:Design
Designed by: Daria Niewiadomska Group: iGEM16_UAM_Poznan (2016-10-12)
pxylS-E1_5'UTR->sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 174
Design Notes
We started working on xylose promoters, because of the cheap inducer-xylose and potentially tight control of expression. Modification of E1-enhancer in 5'UTR gives the highest protein expression levels.
Source
Xylose promoter's sequence comes from E.coli genome, E1 enhancer comes from gene 10 of bacteroophage T7.