Generator

Part:BBa_K2014002:Design

Designed by: Daria Niewiadomska   Group: iGEM16_UAM_Poznan   (2016-10-12)


pxylS-E1_5'UTR->sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


Design Notes

We started working on xylose promoters, because of the cheap inducer-xylose and potentially tight control of expression. Modification of E1-enhancer in 5'UTR gives the highest protein expression levels.


Source

Xylose promoter's sequence comes from E.coli genome, E1 enhancer comes from gene 10 of bacteroophage T7.

References