Generator

Part:BBa_K2012027:Design

Designed by: Boyao Zhang   Group: iGEM16_HZAU-China   (2016-10-07)


PcpcG2-172-B0034-PleD-His(6)Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 212
    Illegal BamHI site found at 1370
    Illegal XhoI site found at 1564
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 628
    Illegal NgoMIV site found at 775
    Illegal AgeI site found at 1111
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 285


Design Notes

PcpcG2-172 is a 172bp truncated version of 238bp full length PcpcG2, which is effective on the reduction of leakiness and increase in dynamics.


Source

PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.(Part:BBa_K2012002)


References