Part:BBa_K2012008:Design

CheZ with fused with a GS linker and GFP in C teminal

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1331
  

Design Notes

CheZ-3*GSlinker-GFP

Source

GFP is amplify from BBa.EOO40 and the CheZ is amplify from E.coli's genome. GS-linker is get from extended overlap PCR.