Coding
Part:BBa_K2012002:Design
Designed by: Pan Chu Group: iGEM16_HZAU-China (2016-09-04)
PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 14
Illegal BamHI site found at 1172 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 430
Illegal NgoMIV site found at 577
Illegal AgeI site found at 913 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 87
Design Notes
codon optimized, and could express protein in Escheriachia coli.
Source
PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain, in its activated form.