Part:BBa_K2009602:Design

sfGFP11 with promoter and RBS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 68
  

Design Notes

If we PCR from GFP directly, we have to mutate it many times and it is more convenient to directly synthese it.

Source

chenical synthesis and link it to BBa_K081005