Coding
Part:BBa_K200030:Design
Designed by: James Chappell Group: iGEM09_Imperial College London (2010-09-20)
Lipase A from B.subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 726
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Lipase A was amplified from the B.subtilis genome 168 by polymerase chain reaction (PCR) using the following primers:
Fwd = GCTCTAGAGATGCAAATGACGGAAGCTACCTG Rev = CTGCAGCGGCCGCTACTAGTATTATTAACTTGATGTAATCATTGCG
Source
Lipase A from B.subtilis