Composite

Part:BBa_K200026

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)

RcsB+RBS+GFP+TT

This intermediate construct contains a GFP reporter to and can be used to assess the activity of the RcsB receiver protein. Different promoters can be attached upstream of the RBS to complete this transcriptional unit.


Usage and Biology

Imperial iGEM2009 developed this part from BBa_K200000 and BBa_I13504 as part of [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1324


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Categories
Parameters
None