Part:BBa_K2000011:Design

hKLF4-dna binding +repressive

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

we get out one illegal site for Pst1.

Source

cgacccgggcgtggcggcgccgggcggcgcgggcggcggcctcctgtatggccgggagtctgcaccccctccgacagctcccttcaacctggcggacatcaacgatgtgagcccctccggcggcttcgtggccgagctcctgcggcccgaattggacccagtgtacattccgccgcagcagccgcagccgccaggtggcgggctgatgggcaagttcgtgttgaaggcgtcgctgagtgcccctggcagcgagtacggcagcccgtcggtcatcagtgttagcaaaggcagcccagacggcagccacccggtggtggtggcgccctacagcggcgggccgccacgcatgtgcccgaagatcaagcaggagactgtctcctcgtgcaccgtcggtcggcccctagaggcccacttgggcactggacctcctctcagcaatggccaccggccacctgcgcacgactttcccttggggcggcagctccccagcaggactaccccgaccctgggtgccgaggaactgctgagcagccgggactgtcatcctgccctgccgctccccccgggcttccatccccaccccgggcccaactaccctcccttcctgcccgaccagatgcagccgcaggtcccaccgctgcattaccaagagctcatgccacccggttcctgcatgccggaggagcccaaaccaaagaggggaaggcggtcgtggccccggaaaaggacggccactcacacttgtgattatgcaggctgcggcaaaacctacacgaagagttctcatctcaaggcacacctgcgcacccacacaggtgagaaaccttaccactgtgactgggatggctgtgggtggaagtttgcccgctcagatgaactgaccaggcactatcgcaaacacactgggcaccgccccttccagtgccagaaatgcgaccgggcattctcgcggtcggaccacctcgccttacatatgaaaaggcacttttaaa