Coding

Part:BBa_K2000010:Design

Designed by: Qin YE   Group: iGEM16_JSNU-China   (2016-10-12)


hKLF4-dna binding


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutant one Pst I site.


Source

aagttcgtgttgaaggcgtcgctgagtgcccctggcagcgagtacggcagcccgtcggtcatcagtgttagcaaaggcagcccagacggcagccacccggtggtggtggcgccctacagcggcgggccgccacgcatgtgcccgaagatcaagcaggagactgtctcctcgtgcaccgtcggtcggcccctagaggcccacttgggcactggacctcctctcagcaatggccaccggccacctgcgcacgactttcccttggggcggcagctccccagcaggactaccccgaccctgggtgccgaggaactgctgagcagccgggactgtcatcctgccctgccgctccccccgggcttccatccccaccccgggcccaactaccctcccttcctgcccgaccagatgcagccgcaggtcccaccgctgcattaccaagagctcatgccacccggttcctgcatgccggaggagcccaaaccaaagaggggaaggcggtcgtggccccggaaaaggacggccactcacacttgtgattatgcaggctgcggcaaaacctacacgaagagttctcatctcaaggcacacctgcgcacccacacaggtgagaaaccttaccactgtgactgggatggctgtgggtggaagtttgcccgctcagatgaactgaccaggcactatcgcaaacacactgggcaccgccccttccagtgccagaaatgcgaccgggcattctcgcggtcggaccacctcgccttacatatgaaaaggcacttttaaa

References