Coding

Part:BBa_K2000006:Design

Designed by: Qin YE   Group: iGEM16_JSNU-China   (2016-10-10)


hKLF4-fl


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 265


Design Notes

we find two illegal sites in this sequence and mutant them.


Source

                                                     at gaggcagcca

     61 cctggcgagt ctgacatggc tgtcagcgac gcgctgctcc cgtccttctc cacgttcgcg

    121 tccggcccgg cgggaaggga gaagacactg cgtccagcag gtgccccgaa taaccgctgg

    181 cgggaggagc tctcccacat gaagcgactt cccccggtgc ttcccggccg cccctacgac

    241 ctggcggcgg cgaccgtggc caccgacctg gagagtggag gagtcggcgc ggcttgcggc

    301 agcagcaacc cggctcttct gccccggagg gagacggagg agttcaatga tctcctggac

    361 ctggacttta tcctctccaa ctcgctgtcc catcaggagt ccgtggccgc cacggtgtcc

    421 tcctcggcat cagcctcatc ctcgtcctcc ccgtcgagca gcggtccagc cagtgcgccc

    481 tccacctgcc gc ttcagcta tccaatccgg gccgggggcg acccgggcgt ggcggcgccg

    541 ggcggcgcgg gcggcggcct cctgtatggc cgggagtctg caccccctcc gacagctccc

    601 ttcaacctgg cggacatcaa cgatgtgagc ccctccggcg gcttcgtggc cgagctcctg

    661 cggcccgaat tggacccagt gtacattccg ccgcagcagc cgcagccgcc aggtggcggg

    721 ctgatgggca agttcgtgtt gaaggcgtcg ctgagtgccc ctggcagcga gtacggcagc

    781 ccgtcggtca tcagtgttag caaaggcagc ccagacggca gccacccggt ggtggtggcg

    841 ccctacagcg gcgggccgcc acgcatgtgc ccgaagatca agcaggagac tgtctcctcg

    901 tgcaccgtcg gtcggcccct agaggcccac ttgggcactg gacctcctct cagcaatggc

    961 caccggccac ctgcgcacga ctttcccttg gggcggcagc tccccagcag gactaccccg

   1021 accctgggtg ccgaggaact gctgagcagc cgggactgtc atcctgccct gccgctcccc

   1081 ccgggcttcc atccccaccc cgggcccaac taccctccct tcctgcccga ccagatgcag

   1141 ccgcaggtcc caccgctgca ttaccaagag ctcatgccac ccggttcctg catgccggag

   1201 gagcccaaac caaagagggg aaggcggtcg tggccccgga aaaggacggc cactcacact

   1261 tgtgattatg caggctgcgg caaaacctac acgaagagtt ctcatctcaa ggcacacctg

   1321 cgcacccaca caggtgagaa accttaccac tgtgactggg atggctgtgg gtggaagttt

   1381 gcccgctcag atgaactgac caggcactat cgcaaacaca ctgggcaccg ccccttccag

   1441 tgccagaaat gcgaccgggc attctcgcgg tcggaccacc tcgccttaca tatgaaaagg

   1501 cacttttaaa

References