Part:BBa_K1998010
HydG
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Overview
This part is composed of the hydG gene. All organisms with [Fe] hydrogenase and sequenced genomes contain homologues of hydE, hydF, and hydG. Within several prokaryotic genomes hydE, hydF, and hydG are found in putative operons with [Fe] hydrogenase structural genes.
Biology & Literature
hydG is a radical S-adenosyl methionine (SAM) enzyme which catalyzes formation of a CO- and CN−-bound iron precursor to the H cluster, the cofactor on which [FeFe] hydrogenases are dependent on [1]. hydG breaks up the substrate tyrosine to yield both CO- and CN—ligands which act as precorsors during H-cluster assembly. It has been suggested that hydG cleaves the Cα–Cβ bond of tyrosine and p-cresol and dehydroglycine are formed as by-products [2]. hydE and hydF are maturases also required for the assembly of an active hydrogenase [3]. The binding site in hydG can accommodate either a [5Fe-5S] or a [4Fe-5S] cluster [4].
Protein information
hydG
Mass: 63.74kDa
Sequence:
MSVPLQCNAGRLLAGQRPCGVRARLNRRVCVPVTAHGKASATREYAGDFLPGTTISHAWSVERETHHRYRNPAEWINEAA
IHKALETSKADAQDAGRVREILAKAKEKAFVTEHAPVNAESKSEFVQGLTLEECATLINVDSNNVELMNEIFDTALAIKE
RIYGNRVVLFAPLYIANHCMNTCTYCAFRSANKGMERSILTDDDLREEVAALQRQGHRRILALTGEHPKYTFDNFLHAVN
VIASVKTEPEGSIRRINVEIPPLSVSDMRRLKNTDSVGTFVLFQETYHRDTFKVMHPSGPKSDFDFRVLTQDRAMRAGLD
DVGIGALFGLYDYRYEVCAMLMHSEHLEREYNAGPHTISVPRMRPADGSELSIAPPYPVNDADFMKLVAVLRIAVPYTGM
ILSTRESPEMRSALLKCGMSQMSAGSRTDVGAYHKDHTLSTEANLSKLAGQFTLQDERPTNEIVKWLMEEGYVPSWCTAC
YRQGRTGEDFMNICKAGDIHDFCHPNSLLTLQEYLMDYADPDLRKKGEQVIAREMGPDASEPLSAQSRKRLERKMKQVLE
GEHDVYL
References
[1] Dinis P, Suess D, Fox S, Harmer J, Driesener R, De La Paz L et al. X-ray crystallographic and EPR spectroscopic analysis of hydG, a maturase in [FeFe]-hydrogenase H-cluster assembly. Proceedings of the National Academy of Sciences. 2015;112(5):1362-1367.
[2] Pilet E, Nicolet Y, Mathevon C, Douki T, Fontecilla-Camps J, Fontecave M. The role of the maturase hydG in [FeFe]-hydrogenase active site synthesis and assembly. FEBS Letters. 2009;583(3):506-511.
[3] Posewitz M, King P, Smolinski S, Zhang L, Seibert M, Ghirardi M. Discovery of Two Novel Radical S-Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase. Journal of Biological Chemistry. 2004;279(24):25711-25720.
[4] Driesener R, Duffus B, Shepard E, Bruzas I, Duschene K, Coleman N et al. Biochemical and Kinetic Characterization of Radical S -Adenosyl- l -methionine Enzyme hydG. Biochemistry. 2013;52(48):8696-8707
| None |