Coding

Part:BBa_K1998007:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-12)


psbMZHWK-OPQR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3063
    Illegal BglII site found at 3392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 570
    Illegal NgoMIV site found at 1819
    Illegal NgoMIV site found at 2465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3456


Design Notes

Genes were codon optimised for bacteria using optimiser tool. Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence.



Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References