Coding

Part:BBa_K1998006:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-12)


psbOPQR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1998
    Illegal BglII site found at 2327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 754
    Illegal NgoMIV site found at 1400
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2391


Design Notes

A premature stop codon was found in pubs and sequence was adjusted.


Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References