Coding

Part:BBa_K1998003:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-12)


psbD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence had to be adjusted to allow for 1 bp correction to the BBa suffix ﴾G>C﴿ so that the SpeI cut site is intact. Lac promoter at the start of the operon. RBS are present before the start of the gene. Double terminator present at the end of the operon.

Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References