Composite

Part:BBa_K1997024:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


P+R->sHRP-N->dCas9->Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 415
    Illegal NheI site found at 2038
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 657
    Illegal BamHI site found at 4317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

dCas9 was codon optimized for expression in E.coli

Source

This part is assembled from K1997000 and a dCas9 fragment


References