Part:BBa_K1997020:Design

P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 279
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 986
  Illegal BsaI.rc site found at 340
  Illegal BsaI.rc site found at 1049
  

Design Notes

We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced

Source

This part was synthesized by IDT